1R01DK104828-01A1
Integrative Genetic and Genomic Analyses in the Inflammatory Bowel Disease
The primary goal of this proposal is to elucidate the multidimensional relationships between genetic variation, regulation of host gene expression, the enteric microbiota, and the range of IBD phenotypes to better understand molecular and environmental contributions to the disease.
R01-ES024983
Interpreting molecular role of DNA variants associated with Crohn’s Disease through integrative analysis of open chromatin, epigenome and transcriptome data in diverse and relevant tissues and cells
This project aims to integrate multiple types of functional genomic data from affected and unaffected patients to uncover regions associated with disease. These regions will then be annotated using these data along with epigenetics data generated by the Roadmap Epigenomics Project consortium to interpret how associated variants contribute to disease.
Crohns and Colitis Foundation of America (CCFA) Career Development Award
Epigenetic Regulation of Cytokines in Inflammatory Bowel Disease
Loss of intestinal macrophage tolerance to the enteric microbiota is a central event in the pathogenesis of CD. Using FAIRE to define a suite of open chromatin sites that regulate genes in macrophages isolated from wild type and colitis prone IL-10 deficient mice born and raised germ free and then colonoized with the enteric microbiota we aim to identify elements that drive tolerance.
School Of Medicine Team Translational Science Award
Identifying Genetic- and Microbial-based Molecular Markers of Inflammatory Bowel Disease
Generating chromatin, transcriptome and microbial data sets from one set of biopsy samples obtained from genotyped patients with Crohn’s disease our aim is to unravel new mechanisms that drive the pathogenesis of Inflammatory Bowel Disease.
University Research Council Award
Genome wide identification of DNA regulatory elements that drive human intestinal macrophage
Applying chromatin mapping technology to human lamina propria macrophages isolated from the normal human colon.
NIH/NIDDK PO1
Host Innate Immune-Microbial Interactions in Intestinal Inflammation
The aim of this dynamic program project grant is to study host microbial interactions in the murine experimental colitis and human IBD. Our lab established and is currently running the “Tissue and Genomics” Core for this Program Project. Our goal is to incorporate high throughput sequencing technology (FAIRE-seq, RNA-seq and ChIP-seq) in cells and tissues isolated from these systems.
Broad Medical Research Program
Characterizing noncoding DNA variants in Inflammatory Bowel Disease
The aim of this grant is to use open chromatin regions throughout the human genome to identify SNPs that may be associated with increased risk of Crohn’s disease.
Center of Gastrointestinal Biology and Disease Pilot/Feasibility Grant
Identification of open chromatin regions in colon tissue from patients with IBD.
The objective of this grant was to apply a chromatin mapping technology (Formaldehyde Assisted Isolation of Regulatory Elements (FAIRE) for human tissue.
American Gastroenterological Association Research Scholar Award
Epigenetic Regulation of Cytokines in Inflammatory Bowel Disease
The overall purpose of this award is to identify variations in chromatin and active gene regulatory elements in macrophages and to elucidate the functional significance of these regions in maintaining intestinal immune homeostasis.
